Pinealon (10 mg Vial)

Pinealon (10 mg Vial) is listed for qualified laboratory research use only.

• Research category: Longevity / Bioregulator Research
• COA verified
• Third-party tested when available
• For research and laboratory use only

Important: This product is not intended for human consumption, medical use, diagnostic use, or treatment of any disease.

Pinealon Dosage Chart (10 mg Vial)

Pinealon is commonly studied at 1–2 mg once daily in educational research protocols. A 10 mg vial reconstituted with 1.5 mL bacteriostatic water yields a concentration of approximately 6.67 mg/mL, providing the same easy syringe measurements as the 20 mg version.

Research Use Only – Not for Human Consumption

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Reconstitution

Add 1.5 mL bacteriostatic water

Final Concentration

10 mg ÷ 1.5 mL = ~6.67 mg/mL

At this concentration:

• 1 unit (U-100 insulin syringe) = 0.01 mL ≈ 66.7 mcg
• 15 units = 1.0 mg
• 22.5 units = 1.5 mg
• 30 units = 2.0 mg

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Pinealon (10 mg Vial) – Educational Dosage Protocol

Pinealon is a synthetic tripeptide bioregulator (Glu-Asp-Arg) developed as part of Professor Vladimir Khavinson’s peptide research program. It has been investigated for its potential neuroprotective properties, support of cognitive function, and influence on gene expression within nervous tissue.

This educational protocol outlines a once-daily subcutaneous research schedule using a practical dilution that allows accurate measurements with a standard U-100 insulin syringe.

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Dosing & Reconstitution Guide

Standard Approach (1.5 mL = ~6.67 mg/mL)

Days	Daily Dose	U-100 Syringe
Days 1–5	1.0 mg (1,000 mcg)	15 units (0.15 mL)
Days 6–14	1.5 mg (1,500 mcg)	22.5 units (0.225 mL)
Days 15–20	2.0 mg (2,000 mcg)	30 units (0.30 mL)

Frequency: Once daily via subcutaneous injection, preferably in the morning or early afternoon.

Standard research cycles typically last 10–20 days, with some protocols extending to 28 days. Additional cycles are commonly separated by 2–3 months.

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Reconstitution Steps

1. Draw 1.5 mL of bacteriostatic water using a sterile syringe.
2. Slowly inject the water down the inside wall of the vial.
3. Avoid foaming.
4. Gently swirl until completely dissolved. Do not shake.
5. Label the vial and refrigerate at 2–8°C (35.6–46.4°F).
6. Protect from light.

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Storage

Lyophilized vial

• Store frozen at −20°C (−4°F) until use.

After reconstitution

• Refrigerate at 2–8°C (35.6–46.4°F).
• Avoid repeated freeze–thaw cycles.

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Supplies Needed

Peptide Vials (10 mg each)

• 10-day cycle: 2 vials
• 20-day cycle: 3 vials
• 28-day cycle: 4 vials

U-100 Insulin Syringes

• 1 injection per day
• 10-day cycle: 10 syringes
• 20-day cycle: 20 syringes
• 28-day cycle: 28 syringes

Bacteriostatic Water (10 mL Bottle)

Each vial requires 1.5 mL.

• 10-day cycle (2 vials): 3 mL
• 20-day cycle (3 vials): 4.5 mL
• 28-day cycle (4 vials): 6 mL

One 10 mL bottle is sufficient for all of the above.

Alcohol Prep Pads

Use:

• One pad for the vial stopper
• One pad for the injection site

Daily requirement:

• 2 alcohol swabs per day

Totals:

• 10-day cycle: 20 swabs
• 20-day cycle: 40 swabs
• 28-day cycle: 56 swabs

A 100-count box is recommended.

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Protocol Overview

Goal

Support neuroprotection, cognitive function, and healthy cellular signaling within nervous tissue in laboratory research settings.

Schedule

Once-daily subcutaneous administration for 10–20 days.

Dose Range

1.0–2.0 mg daily with gradual titration.

Reconstitution

Add 1.5 mL bacteriostatic water to each 10 mg vial, yielding approximately 6.67 mg/mL.

Storage

Freeze lyophilized product. Refrigerate after reconstitution. Avoid repeated freeze–thaw cycles.

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Dosing Protocol

Days 1–5

• 1.0 mg daily
• 15 units

Days 6–14

• 1.5 mg daily
• 22.5 units

Days 15–20

• 2.0 mg daily
• 30 units

Frequency

Once daily via subcutaneous injection.

Preferred Timing

Morning or early afternoon.

Cycle Length

Typically 10–20 days, with optional extension to 28 days.

Repeat Cycles

Every 2–3 months if appropriate for the research protocol.

Important

This guide is provided for educational and laboratory reference purposes only.

Research Use Only. Not for Human Consumption.

Storage Instructions

Proper storage preserves peptide quality.

• Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; minimize moisture exposure.
• Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); prepare aliquots if needed and avoid freeze–thaw.
• Allow vials to reach room temperature before opening to reduce condensation uptake.

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
• Inject slowly; wait a few seconds before withdrawing the needle.
• Document daily dose and site rotation to maintain consistency.
• Effects typically become noticeable after 10–14 days of consistent administration^[6].

How This Works

Pinealon is a synthetic tripeptide that appears to cross cellular and nuclear membranes to interact directly with DNA, thereby regulating gene expression in brain tissue^[4]. Research indicates it reduces reactive oxygen species accumulation and necrotic cell death in neurons while modulating the cell cycle to activate proliferative pathways^[3][8]. Preclinical studies demonstrate neuroprotective effects against oxidative stress, ischemic injury, and cognitive decline, with improvements in memory retention and learning capacity observed in animal models^[9][10]. Clinical trials in humans with traumatic brain injury and cerebral disorders have reported improvements in memory, attention, cognitive function, and emotional stability with daily administration of oral or injectable Pinealon^[6][11].

Potential Benefits & Side Effects

Observations from preclinical and clinical literature.

• Neuroprotective effects through reduction of oxidative stress and preservation of neuronal viability^[3][9].
• Improvements in memory, learning capacity, and cognitive function in both animal and human studies^[6][10][11].
• Modulation of caspase‑3 activity may reduce apoptosis in neurons and other tissues^[12][13].
• Potential support for circadian rhythm regulation and pineal gland function^[14].
• Generally well tolerated; occasional mild injection‑site reactions (redness, itching) may occur with subcutaneous administration^[15].

Lifestyle Factors

Complementary strategies for best outcomes.

• Maintain a balanced diet rich in antioxidants and essential nutrients to support neurological health.
• Engage in regular physical activity and cognitive exercises to reinforce brain plasticity.
• Prioritize consistent sleep schedules and stress management practices.
• Consider timing administration in the morning or early afternoon to optimize cognitive benefits throughout the day.

Injection Technique

General subcutaneous guidance from clinical best‑practice resources^[16].

• Clean the vial stopper and skin with alcohol; allow to dry.
• Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue^[17][18].
• Do not aspirate for subcutaneous injections; inject slowly and steadily^[17].
• Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy^[19].

Important Note

This content is intended for therapeutic educational purposes only and does not constitute medical advice, diagnosis, or treatment.

References