Methylene Blue
Quick summary
A phenothiazine dye with a long history in laboratory work, studied for its redox cycling in the mitochondrial electron transport chain. Research focus: mitochondrial electron transport and redox chemistry. This page leads with SRP's low-and-slow starting dose.
2D structure
Overview
A phenothiazine dye with a long history in laboratory work, studied for its redox cycling in the mitochondrial electron transport chain.
Looking for research material? Simple Research Peptides lists Methylene Blue Capsules – 60 Count for laboratory research use.
Mechanism
Accepts and donates electrons and can bypass complex I/III bottlenecks in the mitochondrial electron transport chain.
Research areas
- mitochondrial electron transport
- redox chemistry
Storage & reconstitution
Supplied in capsule form. Store sealed in a cool, dry place away from light and moisture.
Recommended starting dose (low and slow)
| Starting dose | Oral supplement users describe about 5-20 mg per day, split between morning and early afternoon. This is a ready-made oral liquid whose strength depends on the label (1% is about 0.5 mg per drop, 2% about 1 mg per drop). |
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| Frequency | Once or twice daily, morning to early afternoon; avoid late-day use. |
| Reconstitution | Nothing to reconstitute: sold ready-to-use as an oral liquid, so no bacteriostatic water is needed. Check the percentage strength on the label first. |
Studied higher doses
Studies have used doses up to the amounts below. Higher doses can be dangerous. Whether a person can reach them safely depends on height, weight, activity level, training and pre-existing conditions. We recommend following the lower starting dose above, and contacting SRP with any questions.
| Highest amount reported in studies | A conservative ceiling of about 0.5-2 mg/kg for general low-dose use, with side effects more likely above roughly 7 mg/kg; single research doses of 260-280 mg were used in human brain studies. The approved hospital dose (1 mg/kg intravenous for methemoglobinemia) is a different use. Methylene blue is a MAO inhibitor with serious drug interactions. |
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Route and timing
Route: Oral.
Timing: Pending - to be added by SRP
Cycle length and repeat
Pending - to be added by SRP
Half-life
Approximate, for Bobby's review: Several hours (reported from about 5 to 24 hours).
Side effects
Pending - to be added by SRP
Suggested bloodwork and monitoring
Draft, for Bobby's review: CBC and comprehensive metabolic panel (kidney and liver function, electrolytes) before starting and again after a few weeks.
Cautions
Pending - to be added by SRP
Reconstitution calculator
Math aid only. It converts the numbers you enter; it is not dosing advice. For research use only. Prefilled with this page's vial size, reconstitution and low-and-slow starting dose where known; change any value.
Frequently asked questions
What is Methylene Blue studied for?
In research it is studied for mitochondrial electron transport and redox chemistry. Accepts and donates electrons and can bypass complex I/III bottlenecks in the mitochondrial electron transport chain.
How is Methylene Blue given in research?
Oral. See the dosing frequency block on this page for how often.
What amount should a researcher start with?
This page lists SRP's low-and-slow starting dose in the Recommended starting dose section. Higher amounts that appear in studies are listed separately with their safety context. Start with the lower dose and contact SRP with any questions.
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Last updated: October 19, 2026